Year 2014 outputs
Results of the 2014 research groups
Microscopy and Spectroscopy of Surfaces
• The very slow electron microscopy method has been refined and used, for example, to verify and use new techniques for the preparation of physically and technologically important structures consisting of nanometer nanoscale layers and particles, eg nanostructured catalysts or nanocomposite catalysts for water decomposition, oxidation reaction,
Microscopy and Microanalysis
• Using electron microscopy laboratories to display and analyze samples such as diamond powders, organic pigments, butterfly wing quarries, or displaying a few micrometers of a strong nanocrystalline alpha-Fe layer prepared by low-pressure microwave plasma.
Microscopy for biomedicine
• Enhanced imaging and analysis of biosensors by scanning electron microscopy and optical spectroscopy, eg assessment and quantification of microbial cultures and their biofilms in different environments, and for different methods of sample preparation.
• The group has been incorporated into the Czech-Bioimaging Research Infrastructure project, which is considered to be the national partner of the Euro-Bioimaging project.
• Methods have been developed and developed software for quantitative analysis of MR perfusion and spectral images and for multiparametric segmentation for research and clinical diagnosis of cancer.
• The DCE-MRI perfusion metric analysis method using multichannel blind deconvolution was improved by introducing a more realistic model of impulse bolus response (DCATH) and tested simulatively and clinically.
• Several techniques have been developed to improve the automatic segmentation of tumor tissue in uni- and biparametric MR images using symmetry disturbance and cluster analysis of 2-dimensional histograms.
• Research into the involvement of the brain's talamic nucleus (AN, STN) in various types of executive functions and activation stimuli, links to other brain structures and communication with them.
• Impedance cardiography has been monitored for changes in cardiac output at exercise and the ability to tolerate different levels of exercise in heart transplant patients with heart failure.
Environmental Electron Microscopy
• Studying the surface of ice covered with uranyl nitrate solution by means of environmental scanning electron microscopy and fluorescence spectroscopy
• Study of surface microstructure of hydrogel matrices for multipurpose immobilization of cells by means of environmental scanning electron microscopy
Optical micromanipulation techniques
• Light bundles were shaped into spatial patterns similar to „optical networks“, which were enlightened by micro-objects and light effects of the light sorted by size, shape, or internal structure.
• Raman microspectroscopy was used to visualize and characterize the chemical composition and separation of living algae, the analysis of yeast biofilms and the determination of the effect of antibiotics on individual bacterial cells.
Coherent Lasers and Interferometry
• The implementation of unique experimental systems for the capture and cooling of ions and nanoparticles by laser beams has begun.
• The spectral characteristics of long-lived molecular iodine transitions at wavelengths suitable for fundamental metrology of length were measured.
• Methods of generating a length standard with a direct link between the passive optical resonator length and the repetition frequency of the optical frequency synthesizer have been improved. Cryogenics and superconductivity
• Mechanisms of heat transfer by natural convection in cryogenic 4He for Rayleigh numbers less than 5e15 were experimentally studied.
• An insulating washer with low thermal conductivity and high mechanical stiffness was developed for UHV-SPM low-temperature samples.
• Participation in COMPASS experiments.
• Precision metal nanolayers were deposited on a fiber sensor using surface plasmon resonance.
• The method of calculating all types of parasitic defects of general electron-optical regression systems was used to optimize the lens of the transmission electron microscope.
• Operations of electron lithographs have been improved and phase masks for sensory, dimensional norms, atypical grids and diffraction patterns, structural colors of metallic surfaces are used, for example, for securing documents and nuclear power plants.
• Enhancement of electron nozzles, high voltage sources for electron optics and electron welding methods, eg for vacuum gauge for a 12-pin circular Hypcon connector.
• Design and initial realization of the method of shaping the power laser beam with deformable mirrors of its own design.